We study molecular mechanisms of synaptic plasticity and sensory physiology by combining neurogenetics, electrophysiology, super-resolution microscopy and optogenetics.
Functional nanoscopy of the synaptic active zone. Synapses are specialised intercellular contact sites, which serve as the communication link between neurons and their partner cells. At chemical synapses, calcium-ion influx triggers the fusion of transmitter-laden vesicles with the presynaptic membrane at a specific sub-cellular region termed the active zone. Transmitter substances released by this process then diffuse across the synaptic cleft and are sensed by postsynaptic receptors to convey signal transduction. A hallmark of synaptic transmission is its plasticity, which enables synapses to regulate complex brain processes by filtering, modifying, or integrating information. The details of active zone physiology and how its modulation contributes to synaptic plasticity are, however, barely understood.
By combining genetics with high resolution opto- and electrophysiological methods in Drosophila melanogaster (Ehmann et al., 2014), our research tests the hypothesis that active zone physiology is modified during activity-induced plasticity in vivo. To this end, molecular dynamics are followed at single synapse resolution and neurotransmission is both evoked and measured in an intact, alive, and genetically most amenable organism.
Postsynaptic glutamate receptor dynamics. Hebbian plasticity describes an activity-dependent change in synaptic strength that is input-specific and depends on correlated pre- and postsynaptic activity. Thus, Hebbian plasticity represents a powerful synaptic learning rule that provides an attractive subcellular mechanism for models of higher brain functions.
By engaging Channelrhodopsin-2 to evoke activity at the Drosophila neuromuscular junction in vivo, we found that paired pre- and postsynaptic stimulation increases postsynaptic
sensitivity by promoting synapse-specific recruitment of GluR-IIA-type glutamate receptor subunits into postsynaptic receptor fields. Conversely, GluR-IIA is rapidly removed from synapses whose
activity fails to evoke substantial postsynaptic depolarization (Ljaschenko et al.,
2013). Motivated by these observations, we have teamed up with Markus Sauer and are using
dSTORM (direct stochastic optical reconstruction microscopy) to test the hypothesis that Hebbian plasticity guides synaptic maturation, whereas sparse transmitter release
controls the stabilization of the molecular composition of individual synapses.
Circadian synaptic plasticity. The aim of this project is to obtain a deeper mechanistic understanding of the interplay between the circadian cycle and synaptic plasticity.
Together with Tobias Langenhan and
Georg Nagel we are studying how mechanisms of circadian cycling modulate synaptic contacts, whether this relationship is mutual
in that synaptic properties also determine properties of the circadian clock, and how mechanical stimuli as a non-canonical Zeitgeber are integrated into the circadian network and shape
behaviour. To this end, we are combining neurogenetic approaches with optogenetic actuators and localization microscopy in the central clock network of adult Drosophila
Physiological role of aGPCR. Adhesion-type G protein-coupled receptors (aGPCR) form a large class of seven- transmembrane spanning (7TM) receptors involved in a number of critical developmental, immunological and neuronal processes (aGPCR consortium). However, despite these wide-spread functions, aGPCRs are by far the most poorly understood 7TM receptor class. Neither the general biological and pharmacological properties of aGPCR are known, nor have they been utilized yet in biomedicine.
In collaboration with Tobias Langenhan, we recently discovered that Latrophilin/CIRL, a prototype member of this receptor class, shapes mechanosensation in Drosophila (Scholz et al., 2015). This project has now set out to investigate Latrophilin's mechanism of action in sensory neurons.